Figure 3

The effects of OR on (A) COX-2, (B) iNOS, (C, D) HO-1 in macrophages. The cells were treated with (A, B) LPS alone or with LPS and OR for 24 h and (C, D) OR alone for the indicated periods. Protein levels were determined by Western blot analysis, as described in the Materials and Methods, and were quantitated using a Davinch-chemi^™ Chemiluminescence Imaging System CAS-400SM (Core Bio, Seoul, Korea). mRNA expression was analyzed by RT-PCR. Data represent mean ± SE values of duplicate determinations from three independent experiments. *p < 0.01 and **p < 0.001 were calculated from comparisons with the LPS-stimulation value.