Effect of HCE-EA on iNOS and COX-2 protein and mRNA expression in LPS-stimulated RAW 264.7 macrophages. (A) Cells were treated with the indicated concentrations of HCE-EA plus LPS (1 μg/mL) or LPS alone for 20 h. Protein expression levels of iNOS and COX-2 were determined by Western blotting analysis. β-actin was employed as a loading control. (B) Cells were incubated with the indicated concentrations of HCE-EA LPS (1 μg/mL) or LPS alone for 6 h. mRNA expression levels of iNOS and COX-2 were determined by real-time PCR analysis. The expression level of GAPDH mRNA served as the internal control for the normalization of iNOS and COX-2 mRNA expression. Data are expressed as the mean ± the SD (n = 3 independent experiments *p < 0.05, **p < 0.01, ***p < 0.001 vs. LPS alone (control)).