Inhibitory effects of baicalein (BE) on lysoPC-induced phosphorylation of ERK, JNK and MAPK. Cells were pretreated with BE (0.1 to 10 μM) for 1 h followed by incubation with lysoPC (10 μM) for 10 min. (a, up panel) Baicalein attenuated lysoPC-induced phosphorylation of ERK1/2, JNK and p38, as shown by Western blotting. (b, down panel) The RayBio® Cell-Based MAPK ELISA Kit was used for measuring the relative amount of MAPKs phosphorylation in cultured H9c2 cells. Each value represents the mean ± S.E.M, n = 3–4. #
P < 0.05 versus control; *P < 0.05, **P < 0.01 versus cells exposed to lysoPC alone.