Figure 3
From: Antitumor effect of Kanglaite® injection in human pancreatic cancer xenografts
Effect of KLT on the expression of components involved in the PI3K/Akt/mTOR pathway. A. After treatment with KLT for 21 days, PI3K, Akt, and mTOR mRNA expression levels of tumor xenografts were examined by RT-PCR, and the PCR products were analyzed by electrophoresis on a 2% agarose gel, followed an ethidium bromide stain. B. The expression levels of PI3K, Akt, and mTOR mRNA were unchanged (P > 0.05) in the KLT-treated groups compared with the control group after treatment. C. After treatment with KLT for 21 days, PI3K, Akt, pAkt, mTOR, and p-mTOR expression levels were detected by western blotting. D. After treatment with KLT for 21 days, the pAkt and p-mTOR expression levels were significantly lower than those in the control group (*P < 0.05). The expression levels of PI3K, Akt, and mTOR were the same as those in the control group (P > 0.05).