AVO-b modulates the expression of Bcl-2 family proteins, and promotes translocation of Bax and t-Bid to the mitochondria of HL-60 cells in a dose-dependent manner. Whole cell extracts (50 μg), mitochondrial (30 μg) and cytosolic (50 μg) fractions were prepared from HL-60 cells treated with AVO-b as described above. W.E were probed with antibodies against Bak, Bcl-2 and Bcl-xL proteins. Mit. fractions were immuneblotted with antibodies against t-Bid, Bax and VDAC, while Cyt. fractions were probed with antibodies for Bid and Bax. The same Western blots for the Cyt. fractions and W.E were probed with antibodies to α-tubulin and β-actin, respectively.