Gene expressions and examination of skin tissues from bleomycin-induced mice with different treatments. (1) Drug administration regimens for mouse model establishment. B, bleomycin; P, prevention group; P & T, prevention & treatment group. (2) Relative transcript levels of Col1a2, Col3a1 and Ctgf in skin tissues from bleomycin-induced mice with different treatments in P and P & T group. Sa, saline; B, bleomycin; Y, Yiqihuoxue decoction. A. P group; B. P & T group. The expression level of each gene in saline-treated mice was normalized to 1. *, P <0.05; **, P <0.001. (3) Representative histological analysis of HE and Masson’s trichrome staining of mouse skin tissues with different treatments in high magnifications (100 ×). Four mice were used for each group. A. P group; B. P & T group; C, D, results of analysis of dermal thickening from skin tissues from mouse models with different treatments in P and P & T group, respectively. Bars showed the mean ± SD results of analysis of four mice. *, P <0.05; **, P <0.001.