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Figure 6 | BMC Complementary and Alternative Medicine

Figure 6

From: Inhibition of hematopoietic prostaglandin D2 Synthase (H-PGDS) by an alkaloid extract from Combretum molle

Figure 6

Secondary plots to determine K i for the alkaloids for GST Sigma with CDNB as the substrate. A Non-competitive inhibition was obtained with respect to CDNB, and a re-plot of 1/Vmax versus [I] was used to determine Ki CDNB. In B, a replot of Km/Vmax versus [I] was used to determine KiCDNB for the alkaloids. The X- intercept as indicated by the arrow gave the value -Ki which was used to calculate the Ki and K΄i respectively. These gave values of Ki and Ki′ of 7.7 and 9.2 μg/ml. Correlation coefficients of 0.84 and 0.99 were obtained for plots A and B respectively.

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