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Table 1 List of primers, the primer sequences and the primer melting temperature (Tm)

From: Standardized extract of Ficus deltoidea stimulates insulin secretion and blocks hepatic glucose production by regulating the expression of glucose-metabolic genes in streptozitocin-induced diabetic rats

Gene

Primer probe

Sequence

Annealing Tm°C

GK

Forward

5′-CATATGTGCTCCGCAGGACTAG-3′

61.7

Reverse

5′-CTTGTACACGGAGCCATCCA-3′

PEPCK

Forward

5′-GCAACTTCTCTCGGCTCGTT-3′

62.7

Reverse

5′-TGGCAGTTCTACTGGGCTACAC-3′

G6Pase

Forward

5′-GGATCTACCTTGCGGCTCACT-3′

62.7

Reverse

5′-TGTAGATGCCCCGGATGTG-3′

GLUT 2

Forward

5′-CATCAAAACGTAGAGCACGGTAA-3′

63.4

Reverse

5′-TATGGGCATTTAGTCTGCACGTA-3′

GLUT4

Forward

5′-GCTTGGCTCCCTTCAGTTTG-3′

63.4

Reverse

5′-CCTACCCAGCCAAGTTGCAT-3′

PPAR γ

Forward

5′-CATGAGTTCTTGCGCAGTATCC-3′

63.4

Reverse

5′-AGAGCATTGAACTTGACAGCAAAC-3′

Β-actin

Forward

5′-GCTCTGGCTCCTAGCACCAT-3′

55

Reverse

5′-GCTGATCCACATCTGCTGGAA-3′

  1. GK: Glucokinase, PEPCK: Phosphoenolpyruvate carboxykinase, G6Pase: Glucose-6-phosphatase, GLUT2: Glucose transporter type 2, GLUT4: Glucose transporter type 4, PPARγ peroxisome proliferator-activated receptors-gamma.