Gene | Primer probe | Sequence | Annealing Tm°C |
---|---|---|---|
GK | Forward | 5′-CATATGTGCTCCGCAGGACTAG-3′ | 61.7 |
Reverse | 5′-CTTGTACACGGAGCCATCCA-3′ | ||
PEPCK | Forward | 5′-GCAACTTCTCTCGGCTCGTT-3′ | 62.7 |
Reverse | 5′-TGGCAGTTCTACTGGGCTACAC-3′ | ||
G6Pase | Forward | 5′-GGATCTACCTTGCGGCTCACT-3′ | 62.7 |
Reverse | 5′-TGTAGATGCCCCGGATGTG-3′ | ||
GLUT 2 | Forward | 5′-CATCAAAACGTAGAGCACGGTAA-3′ | 63.4 |
Reverse | 5′-TATGGGCATTTAGTCTGCACGTA-3′ | ||
GLUT4 | Forward | 5′-GCTTGGCTCCCTTCAGTTTG-3′ | 63.4 |
Reverse | 5′-CCTACCCAGCCAAGTTGCAT-3′ | ||
PPAR γ | Forward | 5′-CATGAGTTCTTGCGCAGTATCC-3′ | 63.4 |
Reverse | 5′-AGAGCATTGAACTTGACAGCAAAC-3′ | ||
Β-actin | Forward | 5′-GCTCTGGCTCCTAGCACCAT-3′ | 55 |
Reverse | 5′-GCTGATCCACATCTGCTGGAA-3′ |