Figure 4

Lactoferrin N-lobe purification profile. (A) A typical elution profile of N-lobe lactoferrin on a Mono S 5/50 GL column pre-equilibrated with 50 mM Tris-HCl buffer, pH 8.0 at flow rate 0.7 ml/min and fraction size of 1.0 ml/fraction. (B) 12% SDS-PAGE of the N and C lobes of camel lactoferrin during purification on a Sephadex G-50 column. Lane 1, N lobe, lane 2, C lobe.