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Table 2 Chromosome aberration assay and relative cell counts of Guibi-Tang (GBT) extract

From: Genotoxicity evaluation of Guibi-Tang extract using an in vitro bacterial reverse mutation assay, chromosome aberration assay, and in vivo micronucleus test

Nominal conc. of GBT (μg/mL) S9 mix Time hours a) Mean total aberrant metaphases Mean total aberrations Mean of PP+ER Relative cell counts (%)
6 h treatment (+S9)       
0 + 6-18 0.5/0.5 1.0/1.0 3.0 + 0.0 100
1250 + 6-18 1.0/1.0 2.0/2.0 0.5 + 0.0 96
2500 + 6-18 1.5/1.0 2.0/1.5 1.0 + 0.0 96
5000 + 6-18 2.0/1.5 2.5/2.0 2.0 + 0.0 85
CPA 6 + 6-18 30.0/29.5** 44.0/43.5 1.0 + 0.0 62
6 h treatment (-S9)       
0 - 6-18 0.0/0.0 0.0/0.0 1.0 + 0.0 100
1250 - 6-18 0.0/0.0 0.0/0.0 1.0 + 0.0 94
2500 - 6-18 2.5/2.5 2.5/2.5 1.0 + 0.0 92
5000 - 6-18 0.5/0.5 1.0/1.0 2.0 + 0.0 83
EMS 800 - 6-18 22.5/22.0** 34.0/33.0 0.5 + 0.0 63
22 h treatment (-S9)       
0 - 22-2 0.0/0.0 0.0/0.0 1.0 + 0.0 100
1250 - 22-2 0.0/0.0 0.0/0.0 1.5 + 0.0 94
2500 - 22-2 0.5/0.5 1.0/1.0 0.5 + 0.0 89
5000 - 22-2 2.0/1.5 3.5/3.0 1.0 + 0.0 80
EMS 600 - 22-2 31.5/31.5** 46.0/46.0 1.0 + 0.0 54
  1. **Significantly different from the control at P<0.01.
  2. a) Treatment time-recovery time.