Figure 2

Morphological changes of 1321N1, SW1783 and LN18 cells after treatment. a. Morphological changes of 1321N1 cells after treatment with (i) vehicle untreated cells (ii) GTT 40 μg/ml, (iii) HC 12 μg/ml, (iv) 40 μg/ml of GTT + 12 μg/ml of HC, (v) scale 100 μm; 40 μg/ml of GTT + 12 μg/ml of HC; for 24 h. Arrow shows; x. chromatin margination (cresents), y. the collapse of nuclease and vacuolized cytoplasm, and z. cell shrinkage. b. Morphological changes of SW1783 cells after treatment with (i) vehicle untreated cells (ii) GTT 40 μg/ml, (iii) HC 2 μg/ml, (iv) 40 μg/ml of GTT + 2 μg/ml of HC, (v) scale 100 μm; 40 μg/ml of GTT + 2 μg/ml of HC; for 24 h. Arrow represents; x. collapse of nuclease, y. volume loss and chromatin clumping, and z. cell shrinkage. c. Morphological changes of LN18 cells after treatment with (i) vehicle untreated cells (ii) GTT 20 μg/ml, (iii) HC 29 μg/ml, (iv) 20 μg/ml of GTT + 29 μg/ml of HC, (v) scale 100 μm; 20 μg/ml of GTT + 29 μg/ml of HC; for 24 h. Arrow represents x. volume loss and chromatin clumping, y. collapse of nuclease, and z. cell shrinkage.