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Figure 5 | BMC Complementary and Alternative Medicine

Figure 5

From: 3,4-Dihydroxytoluene, a metabolite of rutin, inhibits inflammatory responses in lipopolysaccharide-activated macrophages by reducing the activation of NF-κB signaling

Figure 5

Effect of DHT on activation of NF-kB pathway in LPS-stimulated RAW264.7 macrophage. RAW264.7 macrophage were pre-treated with DHT for 30 min, followed by stimulating with LPS (100 ng/ml) for 30 min. (A) The phosphorylation and total protein levels of IκB and α-tubulin were determined by Western blot analysis. (B) The p65 subunit of NF-kB in cytoplasm and p65 in nucleus were determined by western blot analysis. RI (Relative intensity) was measured by the densitometry scanning. The data was expressed as the means ± SE from three separated experiments. *P < 0.05, **P < 0.01, significantly with the LPS-treated group only.

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BMC Complementary Medicine and Therapies

ISSN: 2662-7671

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