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Figure 2 | BMC Complementary and Alternative Medicine

Figure 2

From: 3,4-Dihydroxytoluene, a metabolite of rutin, inhibits inflammatory responses in lipopolysaccharide-activated macrophages by reducing the activation of NF-κB signaling

Figure 2

Effect of DHT on inhibition nitric oxide and pro-inflammatory cytokines production in LPS-stimulated RAW264.7 macrophage. RAW264.7 macrophage were pre-treated with DHT for 30 min, followed by stimulating with LPS (100 ng/ml) for 6 and 24 hours. (A) After incubation for 24 h, nitric oxide concentration in culture medium was assayed by the Gress reaction. After incubation for 6 h, the (B) TNF-α and (C) IL-6 mRNA expression and secreted concentration in culture medium was assayed by RT-PCR and ELISA. (D) The levels of IL-1β mRNAs and protein were determined by RT-PCR and ELISsA. The data was expressed as the means ± SE from three separated experiments. *P < 0.05, **P < 0.01, significantly with the LPS-treated group only.

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BMC Complementary Medicine and Therapies

ISSN: 2662-7671

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