Effects of rutin metabolites on NO production in LPS-stimulated RAW 264.7 and DHT cytotoxicity test. (A) After LPS stimulation, RAW264.7 macrophage with rutin or its metabolites including DHT, DHPAA, HPAA and HVA treatment was assessed to NO production measurement. (B) Cell cytotoxicity assay for DHT. RAW 264.7 was treated with DHT for 24 hours, followed by incubating with MTT reagent to measure OD 570 nm by spectrophotometry. The data was expressed as the means ± SE from three separated experiments.