Figure 4

Effect of opioid μ-receptor blockade on rhodiola-water extract-induced changes of protein levels of GLUT 4 in skeletal muscle and hepatic PEPCK in STZ-diabetic rats. (A) The representative response of protein level for GLUT 4 or actin in skeletal muscle isolated from STZ-diabetic rats receiving treatment with rhodiola-water extract only or with cyprodime in combination, three times daily for 3 days. (B) The representative response of protein level for PEPCK or actin in liver isolated from STZ-diabetic rats receiving same treatment with rhodiola-water extract only or with cyprodime in combination. Lane1, vehicle-treated Wistar rats; lane2, vehicle-treated STZ-diabetic rats; lane 3, rhodiola-water extract (75 mg/kg)-treated STZ-diabetic rats; lane 4, rhodiola-water extract (75 mg/kg) plus cyprodime (1.0 mg/kg)-treated STZ-diabetic rats. Quantification of protein level using GLUT 4/actin or PEPCK/actin showing mean with standard error (SE) (n = 6 per group) in each column is indicated in the lower panel. **, p < 0.01 and ***, p < 0.001 compared with data obtained from lane 1. #, p < 0.05, ##, p < 0.01 and ###, p < 0.001 compared with data obtained from lane 2.