Figure 4

Cytokine expression in draining lymph nodes. Mice (n = 5) were treated as shown in Figure 3. DLN were obtained and pooled one week after the third sensitization course. DLN cells were cultured in the presence of 50 μg/mL OVA. Cells were harvested after 24 h for RNA and cDNA preparation. Expression levels of (A) IFN-γ, (B) IL-4, (C) IL-10, and (D) IL-17A were measured using quantitative PCR. Data are represented the mean ± SEM and performed one-way analysis of variance (ANOVA) followed by Tukey’s multiple comparison test. All p-values less than 0.05 were considered statistically significant.