Figure 4

Assessment of apoptosis by Annexin V/PI staining on the human breast adenocarcinoma cell line (MCF-7). The cells were treated with 250 and 500 μg/ml galangal extract for 48 h (symbol II, III) or media (control symbol I), and apoptosis was examined with flow-cytometry after Annexin V-PI double staining. The necrotic cells lost cell membrane integrity that permits PI entry .Viable cells exhibit Annexin V (-)/PI (-); early apoptotic cells exhibit Annexin (+)/PI (-); late apoptotic cells or necrotic cells exhibit Annexin V (+)/PI (+).