Effects of baicalin on IRI induced proinflammatory response. (A) Baicalin inhibition of the IRI induced up-regulation of proinflammatory cytokines. (B, C) Activation of the TLR2/4 signaling pathway by IRI, contributing to a proinflammatory response. Pretreatment with baicalin decreased TLR2, TLR4, and MyD88 expression (B) and suppressed the phosphorylation of NF-κB and IκB (C), thereby inhibiting related proinflammatory signaling pathways. *P < 0.05, **P < 0.01, ***P < 0.001.