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Table 4 Fold change in gene expression measured by gene array and Q-RT-PCR

From: Berberine moderates glucose metabolism through the GnRH-GLP-1 and MAPK pathways in the intestine

Gene symbol

BerL vs DM

BerH vs DM

Fold change (gene array)

Fold change (Q-RT-PCR)

P _value (Q-RT-PCR)

Fold change (gene array)

Fold change (Q-RT-PCR)

P _value (Q-RT-PCR)

Glp1r

3.862

4.6 ± 0.3

0.027

4.231

4.3 ± 0.2

0.026

Gnrh1

−4.615

−3.7 ± 0.2

0.038

−3.758

−3.5 ± 0.3

0.025

Gnrhr

−3.715

−3.1 ± 0.4

0.014

−3.271

−2.9 ± 0.4

0.039

Mapk10

6.362

5.9 ± 0.3

0.031

5.837

6.2 ± 0.5

0.015

  1. Glp1r: glucagon-like peptide 1 receptor; Gnrh1: gonadotropin-releasing hormone 1; Gnrhr: gonadotropin releasing hormone receptor; Mapk10: mitogen activated protein kinase 10.
  2. Each Q-RT-PCR assay was repeated using three biological replicates and each analysis consisted of three technical replicates.