The seed extract did not caused apoptosis on MCF-7 cells. (A) Nuclear morphology analysis by DAPI stain. The seed extract caused a few nuclear alterations such as nuclear shrinking (arrow). There was no evidence of apoptosis. (B) Caspase-Glo® 3/7 luminescent demonstrated that none of the extracts increased the activity of caspase 3 and caspase 7 when compared to control cells after 24 h of treatment.