Figure 5

The effects of ethanol extract of Saussurea lappa (ESL) on the 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced mitogen-activated protein kinase (MAPK) signaling pathway in MCF-7 cells. Cells were pretreated with TPA for 15 min in the presence or absence of ESL. (A) Cell lysates were prepared for western blotting with specific p-p38, p38, c-Jun N-terminal kinase (JNK), phosphorylated-JNK, extracellular signal-regulated kinase (ERK), and phosphorylated-ERK antibodies. (B) Quantitative results of phosphorylation levels of p38, ERK and JNK were adjusted with total p38, ERK and JNK protein level.