Figure 3From: Saussurea lappa extract suppresses TPA-induced cell invasion via inhibition of NF-κB-dependent MMP-9 expression in MCF-7 breast cancer cells The effect of ethanol extract of Saussurea lappa (ESL) on the 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced nuclear factor-kappa B (NF-κB) activation in MCF-7 cells. MCF-7 cells were treated with ESL in the presence of TPA. Following 3 h of incubation, nuclear extracts were prepared as described in Methods. (A, B) NF-κB/activator protein-1 (AP-1) DNA binding was analyzed by electrophoretic mobility shift assay as described in Methods.Back to article page