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Figure 5 | BMC Complementary and Alternative Medicine

Figure 5

From: Enhancement of gefitinib-induced growth inhibition by Marsdenia tenacissima extract in non-small cell lung cancer cells expressing wild or mutant EGFR

Figure 5

MTE ( M. tenacissima extract) enhances the inhibition of gefitinib on PI3K/Akt/mTOR and ERK1/2 signaling cascade in non-small cell lung cancer cells. H292 and HCC827 cells were treated with 1 μM gefitinib, 8 mg/ml MTE, and their combination (MTE → MTE + Gef, M → M + G) in 1% FBS culture medium for 6 h. Cells were stimulates with 10 ng/ml EGF for 15 min before harvest. Cells were lysed and cellular extracts (20 μg protein) were separated on SDS-PAGE gel and transferred to PVDF membranes. (A) Membranes were probed with PI3K, phospho-PI3K, Akt and phospho-Akt; (B) Membranes were probed with mTOR, phospho-mTOR, ERK1/2 and phospho-ERK1/2. Blots are representatives of three independent experiments. Relative density of proteins were calculated and normalized based on β-tubulin or β-actin.

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