Figure 7
Expression of Bcl-2, Bcl-3, Bax and Bim mRNA in lymph nodes 48 h post SEB injection. Balb/c mice (n = 3 per group) were treated as described in the Materials and Methods. (A) mRNA from naïve, SEB or SEB + PPC treated mice was analyzed for the expression of Bcl-2, Bcl-3, Bax, and Bim, while (B) expression of the same genes in mice treated with PPC only were analyzed. Total cell RNA was pooled from the lymph nodes of Naïve, SEB and SEB + PPC treated mice and then converted to ssDNA. Duplicate samples from each group were used for RT-PCR. The fold change in RNA levels for each gene was determined using the ∆∆-Ct method and β-actin as the expression control.