FLP inhibits NF-κB translocation activity and EMT- associated protein in vitro . Effect of FLP on NF-κB expression and EMT-associated proteins in A549 cells. (A) Western blot analysis of cytoplasmic and nuclear expression of NF-κB when A549 cells were cultured with or without macrophages, +/- FLP treatment, NRS (vehicle control). (B) Immunofluorescent staining of NF-κB localization following 24-hour of treatment counterstained with DAPI, representative pictures at original magnification × 400 were shown. (C) Western blot analysis of total protein for Snail, Slug, ZEB1 expression. (D) Western blot analysis of total protein E-cadherin, N-cadherin and Vimentin expression. (E) Western blot analysis of total protein MMP2 and MMP9 levels. All the experiments were repeated 3 times.