FLP inhibits A549 cell proliferation and migration in vitro. Effect of FLP on cellular proliferation and migration. (A) A549 cells were seeded in 96-well plates and treated with NRS (vehicle control), FLP treatment (low, medium, high) or CTX for 48 hours and subjected to MTT assay. Data are shown as mean ± standard error for three independent experiments. *P < 0.05 versus NRS. (B) A549 cells were co-cultured with THP-1 macrophages for 24 hours and treated with FLP at different dosages and subjected to scratch-wound healing assay. Representative photomicrographs of three independent experiments are shown and quantitated using imaging software measured as pixels ( C ). *P < 0.05 versus NRS.