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Table 1 Effects of Polygonum viviparum (PV) on cyclic guanosine 3′, 5′-monophosphate (cGMP) formation by the rat aorta

From: Polygonum viviparum L. induces vasorelaxation in the rat thoracic aorta via activation of nitric oxide synthase in endothelial cells

 

cGMP (pmol/mg protein)

 

Intact

Denuded

l-NAME 50 μM

MB 10 μM

Ca2+free medium

Control

0.53 ± 0.08

–

–

–

–

SNP 10 μM

3.45 ± 0.56*

–

–

–

–

ACh 10 μM

3.21 ± 0.42*

0.60 ± 0.18

0.70 ± 0.09

0.52 ± 0.12

0.45 ± 0.11

PV 100 μg/ml

3.18 ± 0.74*

0.57 ± 0.13

0.54 ± 0.09

0.66 ± 0.07

0.64 ± 0.04

  1. After pretreatment with 10 μM IBMX for 5 min, aortic segments were replaced in Ca2+-free Krebs’ (2.5 mM EGTA) buffer or treatment with inhibitors of l-NAME and methylene blue (MB) for 10 min and then sodium nitroprusside (SNP), acetylcholine (ACh) and PV were added for another 2 min. The aortic rings were immersed in liquid nitrogen to stop the reaction. cGMP formation were measured. Data are expressed as the mean ± standard error of the mean (S.E.M) of more than 5 individual experiments. –, not determined. * p < 0.05 indicates a significant difference from the control.
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BMC Complementary Medicine and Therapies

ISSN: 2662-7671

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