Smad3 phosphorylation in diabetic mice was suppressed by astragalus injection. Smad3 was actived in diabetes. The ratio of phosphorylated Smad3 to total Smad3 was increased in KKAy mice. In addition, Smad3 activation was significantly inhibited in diabetic mice treated with astragalus. Representative western blots of p-Smad3 and total Smad3 are shown. CG = the control group, MG = the model group, and TG = the astragalus group. Compared with CG, *P < 0.05, **P < 0.01. Compared with MG, Δ
P < 0.05,
P < 0.01.