Renal pathology of the different groups. Pathological damage in KKAy mice at 24 weeks of age. Glomerulosclerosis and interstitial fibrosis are the main pathology in diabetic nephropathy. A-C: HE staining, 400X. The CG (A), the MG (B), and the TG (C). HE staining revealed a thickened basal membrane and vacuolar degeneration in the renal tubular epithelial cells. D-F: Masson staining, 400X. The CG (D), the MG (E), and the TG (F). Masson staining revealed collagen deposition (blue color) in the interstitium and glomeruli, which indicated improved renal pathology.