Angelica Sinensis (AS) induced myotube hypertrophy after 72 h treatment. Histograms of myotube diameters derived from microscope images. Right panel shows a representative myotubes of image. Scale bar = 50 μm. Quantification of mean myotube diameter (M) ± SD. (A) NON, non- Angelica Sinensis supplements, Dulbecco’s modified Eagle’s medium (DMEM) containing 2% horse serum (HS), n = 107; (B) AS, 10 ng/mL of AS in 2% HS/DMEM, n = 91; n-value represented the myotube numbers from image. The mean of myotube diameters (M) was significantly enlarged in AS group. Data were analyzed with the Student’s t-test. Statistical significance: *P < 0.05 vs. NON.