Figure 4

Effects of TCE on the MAPKs pathway and PI3K/Akt signaling. Huh7 cells were cultured in various concentrations of TCE (0, 25, 50, 75, and 100 μg/mL) for 24 h. The cell lysates were then subjected to SDS–PAGE followed by western blots with (A) anti-ERK1/2, (B) anti-JNK, (C) anti-p38, and (D) anti-Akt (total and phosphorylated) antibodies, as described in the Methods Section. The activities of these proteins were subsequently quantified by densitometric analyses, with the control being 100% (shown just below the gel data). The values in this figure represent the means ± SD of at least 3 independent experiments. *p < 0.05 as compared with the vehicle group.