Figure 1

Effects of TCE on the Protein Levels of u-PA and its Endogenous Inhibitor PAI-1. (A-B) Huh7 cells were treated with TCE (0–100 μg/mL) for 24 h and then subjected to casein zymography to analyze the activity of u-PA. (C) Huh7 cells were treated with TCE (0–100 μg/mL) for 24 h and then subjected to western blotting to determine the protein levels of u-PA and PAI-1. (D) Quantitative results of u-PA and PAI-1 protein levels, which were adjusted with the β-actin protein level. The values in this figure represent the means ± SD of at least 3 independent experiments. *p < 0.05 as compared with the vehicle group.