Evaluation of the diuretic activity of the aqueous and 80% methanol extracts of Ajuga remota Benth (Lamiaceae) leaves in mice

Table 2 Effect of aqueous and 80% methanol extracts of the leaves of Ajuga remota on 5 h urinary electrolyte excretion in mice

Group	Urinary electrolyte concentration (mmol/L)			Saluretic index			Na⁺/K⁺	Cl⁻/ Na⁺+K⁺
	Na⁺	K⁺	Cl⁻	Na⁺	K⁺	Cl⁻
DW	59.97 ± 7.83	43.7 ± 5.80	44.2 ± 2.58				1.37	0.43
F10	101.3 ± 6.01^a3	92.07 ± 13.85^a3	73.85 ± 11.27	1.68	2.1	1.67	1.10	0.38
AA250	49.70 ± 2.74^b3,c3	57.5 ± 5.94^b1	50.87 ± 10.58	0.83	1.32	1.13	0.86	0.47
AA500	80.62 ± 4.89	72.85 ± 2.66^c1	58.75 ± 8.34	1.34	1.67	1.33	1.11	0.38
AA1000	101.5 ± 6.98^a3	38.08 ± 1.81^b3	83.37 ± 2.71^a3	1.69	0.87	1.87	2.66	0.59
AM250	63.75 ± 7.3^b1,d1	97.5 ± 13.2^c1	55.74 ± 6.23	1.06	2.23	1.26	0.65	0.34
AM500	90.37 ± 5.8^a1,e1	100.6 ± 11.7^c2	56.82 ± 8.06	1.51	2.30	1.29	0.89	0.29
AM750	101.7 ± 4.72^a2,e2	53.5 ± 2.95	77.78 ± 9.71^a1	1.69	1.22	1.76	1.90	0.50

(n = 8); For aqueous extract ^aagainst control, ^bagainst standard, ^cagainst AA1000 mg/kg; For 80% methanol extract, ^aagainst control, ^bagainst standard, ^cagainst AM750 mg/kg, ^dagainst AM500 mg/kg, ^eagainst AM250 mg/kg; ¹p < 0.05, ²p < 0.01, ³p < 0.001; Saluretic Index = mmol of electrolyte of test group/mmol of electrolyte of control group; AA refers to aqueous extract and AM to 80% methanol extract of Ajuga remota; F10: Furosemide 10 mg/kg CON: controls treated with distilled water; Numbers refer dose in mg/kg.

ISSN: 2662-7671