Evaluation of the diuretic activity of the aqueous and 80% methanol extracts of Ajuga remota Benth (Lamiaceae) leaves in mice

Table 1 Effect of aqueous and 80% methanol extracts of the leaves of Ajuga remota on 5 h urine volume in mice

Group	Volume of urine (ml)					Diuretic action	Diuretic activity
	1 h	2 h	3 h	4 h	5 h
CON	0.62 ± 0.13	0.81 ± 0.11	0.97 ± 0.11	1.0 ± 0.11	1.05 ± 0.10	1.0
F10	1.18 ± 0.07^a1	1.46 ± 0.09^a3	1.64 ± 0.08^a1	1.79 ± 0.09^a2	2.05 ± 0.11^a2	1.95	1.0
AA250	0.38 ± 0.04^b3,c3	0.69 ± 0.05^b3,c2	0.98 ± 0.17^b2	1.11 ± 0.18^b2,c1	1.16 ± 0.17^b3,c2,d1	1.10	0.56
AA500	0.56 ± 0.08^b3,c2	1.09 ± 0.07	1.41 ± 0.12	1.68 ± 0.13^a1	2.03 ± 0.13^a2	1.93	0.99
AA1000	1.13 ± 0.13^a2,g3,h3	1.26 ± 0.14^a1,f3,g3	1.54 ± 0.19^a1	1.73 ± 0.17^a2	2.06 ± 0.20^a2	1.96	1.01
AM250	0.45 ± 0.46^b3,c2	0.62 ± 0.07^b3	0.68 ± 0.72^b3,c2	0.84 ± 0.07^b3,c2	1.1 ± 0.85^b3,c3	1.06	0.54
AM500	0.48 ± 0.09^b3,c1	0.71 ± 0.09^b3	0.91 ± 0.12^b3	1.08 ± 0.13^b2,c1	1.31 ± 0.11^b2,c2	1.25	0.64
AM750	0.86 ± 0.03^e3,d3	0.98 ± 0.08^e3	1.32 ± 0.13	1.57 ± 0.16^a1	2.01 ± 0.19^a2	1.92	0.98

(n = 8); For aqueous extract: ^aagainst control, ^bagainst standard, ^cagainst AA1000 mg/kg, ^dagainst AA500 mg/kg, ^eagainst AA250, ^fagainst AM250, ^gagainst AM500, ^hagainst AM750; For 80% methanol extract ^aagainst control, ^bagainst standard, ^cagainst AM750 mg/kg; ¹p < 0.05, ²p < 0.01, ³p < 0.001; AA refers to aqueous extract and AM to 80% methanol extract Ajuga remota; F10: Furosemide 10 mg/kg CON: controls treated with distilled water; Numbers refer dose in mg/kg; Diuretic activity is considered to be good if it is more than 1.50, moderate if it is 1.00-1.50, little if it is between 0.72-1.00 and nil if it less than 0.72.

ISSN: 2662-7671