Table 1 Purification scheme for meswak α-amylase
From: Purification and characterization of α-Amylase from Miswak Salvadora persica
Step | Total protein (mg) | Total activity (units)* | Specific activity (units/mg protein) | Fold purification | Recovery % |
|---|---|---|---|---|---|
Crude extract | 19.77 | 4908 | 248 | 1.0 | 100 |
Chromatography on DEAE- Sepharose | |||||
0.0 M NaCl (A1) | 1.83 | 1805.7 | 986.72 | 3.97 | 36.8 |
0.05 M NaCl (A2) | 1.16 | 488.49 | 421.11 | 1.69 | 9.95 |
0.1 M NaCl (A3) | 1.59 | 437.96 | 275.45 | 1.11 | 8.93 |
0.2M NaCl (A4) | 2.48 | 647.62 | 261.14 | 1.05 | 8.92 |
0.3M NaCl (A5) | 2.26 | 689.75 | 305.2 | 1.23 | 14.05 |
0.4 M NaCl (A6) | 1.48 | 479.24 | 323.8 | 1.30 | 9.76 |
Gel filtration on Sephacryl S-200 | |||||
α-Amylase A1 | 0.1 | 1021 | 10210 | 41 | 20.8 |
α-Amylase A4a | 0.12 | 163.3 | 1385 | 5.4 | 3 |
α-Amylase A4b | 0.063 | 351 | 5571 | 22 | 7 |
α-Amylase A5a | 0.2 | 300 | 1500 | 6 | 6.1 |
α-Amylase A5b | 0.03 | 302 | 10066 | 40 | 6 |