Figure 3

Effects of EEPC on LPS-induced IL-1β and TNF-α release in RAW 264.7 macrophages. Cells were pre-treated with the indicated concentrations of EEPC 1 h prior to incubation with LPS (0.5 μg/ml). After incubation for 24 h, the levels of IL-1β (A) and TNF-α (B) present in the supernatants were measured using ELISA kits. The values shown here are means ± SD of three independent experiments. * P < 0.05 indicates a significant difference from the value obtained for cells treated with LPS in the absence of EEPC.