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Figure 2 | BMC Complementary and Alternative Medicine

Figure 2

From: Ethanol extract of Poria cocos reduces the production of inflammatory mediators by suppressing the NF-kappaB signaling pathway in lipopolysaccharide-stimulated RAW 264.7 macrophages

Figure 2

Down-regulation of LPS-induced iNOS and COX-2 protein and mRNA expression by EEPC pre-treatment in RAW 264.7 macrophages. (A) Cells were pre-treated with different concentrations of EEPC for 1 h, then with LPS (0.5 μg/ml), and incubated for 24 h. Total cellular proteins (30 μg) were resolved by SDS-polyacrylamide gel electrophoresis, transferred to nitrocellulose membranes, and detected with anti-COX-2 and iNOS antibodies, and an ECL detection system. (B) After LPS treatment for 6 h, total RNA was prepared for the RT-PCR analysis of COX-2 and iNOS gene expression. The amplified PCR products were run in a 1% agarose gel and visualized by EtBr staining. Actin and GAPDH were used as internal controls for the Western blot and RT-PCR assays, respectively.

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