Dermatitis scores and histological changes in D. farinae extract-sensitized Nc/Nga mice. A: Macroscopic changes following consecutive administration of A. capillaris extract (AC) or Protopic ointment to D. farinae-induced AD like lesions on the back and ears in Nc/Nga mice. The images show the back and ears 4 weeks after sensitization. B: The dermatitis scores of D. farinae-induced AD-like lesions on the back and ears (mean ± SEM (n = 5), *: P < 0. 05 and **: P < 0. 01, compared with the normal group, ##: P < 0. 01, compared with the D. farinae-induced control group). AC (10 mg/mouse/day) and Protopic ointment (50 mg/mouse/day) were topically applied on the back and ears once daily for 4 weeks. C: Histological features of the back and ears. The tissues were stained with hematoxylin & eosin (H&E) or toluidine blue to estimate epidermal inflammation or mast cell infiltration. The mast cells are indicated by arrows.