Hair growth-promoting effects of Thuja orientalis extract. (a) Telogen-matched, 6-week-old C57BL/6 N mice were shaved and topically applied with control (n = 10; A–F), T. orientalis extract (5.05 mg/cm2/day; n = 10; G–L), or 1% minoxidil (n = 10; M–R). The back skins were photographed at 0, 7, 10, 14, 17, and 21 days after depilation. The image shown is a representative picture of 10 mice. Bars, 100 μm. (b) Hair lengths at different time intervals after treatment with T. orientalis extract. Data shown represent means ± standard deviation (S.D.) of 3 independent experiments. Comparisons of multiple group means were performed using One-way ANOVA, followed by the Dunnett’s t-test (***
p < 0.001, vs. control; ###
p < 0.001, vs. 1% minoxidil).