Expression of podocalyxin in the kidney after STZ injection in rats. Kidney histology and expression of podocalyxin were documented with HE staining, immunofluorescent staining of podocalyxin and RT-PCR analysis of podocalyxin mRNA respectively. As shown in panel A, glomerulus was damaged and podocalyxin fluorescent intensity was reduced (magnification x400). The expression of podocalyxin was gradually reduced from 0 to 12 weeks after STZ injection. The expression of podocalyxin was further documented by RT-PCR analysis of podocalyxin mRNA as displayed in panel B. Podocalyxin level at 12 weeks after STZ injection was about one third of normal value. Data are expressed as mean ± SD from eight rats. * indicates p < 0.05 as compared to normal control group.