P2Et fraction decreases 4T1 cell nuclear segmentation and clonogenic activity. (a) 4T1 cells treated with P2Et fraction for 48 h and stained with DAPI. White arrows show apoptotic cells or fragmented nuclei observed under a fluorescence microscope (40×). (b) 4T1 cells treated with P2Et fraction, ethanol (negative control), and doxorubicin (positive control). Data represent the mean number of colonies ± SEM of three independent experiments (***p < 0.001) versus control.