Lesion size and immunofluorescence intensity A-F. GFAP immunostaining. G. Lesion size. H. Immunofluorescence intensity. The lesion area is delineated along the inner boundary of the GFAP immunostained astrocytic scar (dash line). The immunofluorescence was evaluated in the areas 0.5 mm rostral and caudal to the lesion (solid line). In B the ventral part of the spinal cord was broken. A straight line between the broken ends was drawn (arrow). Also, at the upper right and left parts (arrow heads) there were two small parts where the solid line was drawn outside of the section. The areas of these two small parts were measured. They covered less than 1% of the entire interested area, and were neglected in the evaluation. A-D. LBP-pre group: A, C. 7 d and 14 d vehicles; B, D. 7 d and14 d corresponding LBP treated cases. At both 7 d and 14 d the lesion sizes were significantly larger than that of the control (G), but there was no significant difference in the immunofluorescence intensity of GFAP (H). E, F. LBP-aft 14 d group. Unlike the LBP-pre group, the lesion area was smaller in the drug group. Similar to the LBP-pre group there was no significant difference in the immunofluorescence intensity between the control and LBP treated groups. *p < 0.05, compared to the corresponding vehicle control. Bar = 1 mm.