Figure 3

Differentiation-specific U937 monocytic cells macrophage cell surface marker expression and cell viability determination. U937 monocytic cells were induced to differentiate with various concentrations of PMA for 24 and 48 hours. (A) The percentages of differentiated cells were scored by the presence of CD11b on the cell surface. (B) The percentages of viable cells were scored using trypan blue exclusion assay. The percentage of differentiated or viable cells per total cells is the same prior to the experiment using the different concentrations of PMA and incubation period. Data are presented as mean ± S.D. from three independent experiments with ‘*’, p < 0.05 represent significance difference in comparison to the group that were not induced to differentiate.