Figure 4

Effect of resveratrol on Akt, JNK, and PAK expression in mammary tumors. Total lysates of MDA-MB-231-derived mammary tumors from vehicle- and Res-treated mice were western blotted with anti-Akt, anti-JNK, or anti-PAK1 antibodies. Fold changes in protein expression were calculated from integrated density of positive bands and are presented relative to vehicle. Equal total protein content as well as confirmation of human cancer cells in the mouse tumor extracts was maintained by expressing the integrated density of each band as a function of the integrated density of the GFP band from the same tumor extract. (A), Representative western blots. (B), (C), and (D), Relative Akt, JNK, and PAK1 expression, respectively. Error bars represent SEMs from 2-4 tumors per group. Differences between means were determined using Student’s t-Test.