Chromatographic separation and fractionation of Ardisia crispa roots. Column chromatographic separation was repeated three times to collect QRF (38.38% w/w). A–E represent the five major fractions obtained using the hexane:ethyl acetate gradients. Fraction 1: AC-2 Rf 0.76 in fractions C and D (vials 91–150). The mixture of fractions C and D obtained in fraction 1 was separated to yield fraction 2. Fraction 2: AC-2 Rf 0.76 in fractions C and D (vials 56–88). The mixture of C and D from fraction 2 was separated again to yield QRF.