Anti-protozoal activity of aporphine and protoberberine alkaloids from Annickia kummeriae (Engl. & Diels) Setten & Maas (Annonaceae)

Table 1 Anti-plasmodial activity (IC ₅₀ ) and cytotoxicity (CC ₅₀ ) of HSCCC fractions of Annickia kummeriae methanolic leaf extract

Fraction	Wt (mg)	P. falciparum K1 IC₅₀(μg/ml)	Cytotoxicity CC₅₀(μg/ml)	SI	IC₅₀fr. IC₅₀CQ	IC₅₀fr. IC₅₀Art	IC₅₀fr. IC₅₀Pdx
AKLM	15,000	0.12 ± 0.01	30.0 ± 0.8	250	1.9	60	3,333
AKLM1	562	5.0 ± 0.31	>90.0	>18	79.4	2500	>10,000
AKLM2	2,999	0.87 ± 0.1	20.0 ± 3.3	23	13.8	435	2,222
AKLM3	1,534	3.01 ± 0.81	78.0 ± 5.4	26	47.8	1,505	8,667
AKLM 4	157	1.34 ± 0.33	76.0 ± 1.44	57	21.3	670	8,444
AKLM 5	249	3.6 ± 0.2	>90.0	>25	57.1	1,800	>10,000
AKLM 6	427	0.45 ± 0.15	59.0 ± 1.5	131	7.1	225	6,556
AKLM 7	165	0.11 ± 0.02	>90.0	>818	1.7	55	>10,000
AKLM 8	221	0.09 ± 0.04	>90.0	>1,000	1.4	45	>10,000
AKLM 9	680	0.06 ± 0.02	>90.0	>1,500	1.0	30	>10,000
AKLM 10	1,295	0.05 ± 0.02	>90.0	>1,800	0.8	25	>10,000
AKLM 11	1,679	0.05 ± 0.01	>90.0	>1,800	0.8	25	>10,000
AKLM 12	1,056	0.06 ± 0.03	>90.0	>1,500	1.0	30	>10,000
AKLM 13	878	0.62 ± 0.4	>90.0	>145	9.8	310	>10,000
AKLM 14	948	1.0 ± 0.22	>90.0	>90	15.9	500	>10,000
AKLM 15	1,232	0.13 ± 0.02	>90.0	>692	2.1	65	>10,000
AKLM 16	416	0.09 ± 0.03	84.0 ± 3.91	933	1.4	45	9,333
AKLM 17	498	1.67 ± 0.43	>90.0	>54	26.5	835	>10,000

Fr. – HSCCC fraction of Annickia kummeriae leaves methanolic extract, P. falciparum K1 used for anti-plasmodial assays, rat myoblast L-6 cells used for cytotoxicity assays, CQ chloroquine (IC₅₀ 0.063 ± 0.034 μg/ml), Art artemisinin (IC₅₀ 0.002 ± 0.00001 μg/ml), Pdx podophyllotoxin (IC₅₀ 0.009 ± 0.003 μg/ml).

ISSN: 2662-7671