Figure 5
From: Capsaicin induces cell cycle arrest and apoptosis in human KB cancer cells
Apoptosis of KB cells is mediated through the activation of caspases. The effect of capsaicin on the activation of caspases and PARP was analyzed by immunoblotting as described in the Methods. Equal amounts of each sample were loaded on SDS-PAGE gels. The expression of caspase 3, 8 and 9, PARP, and β-actin were detected using specific antibodies. Equal protein loading was confirmed by stripping and reprobing the blot to detect β-actin expression. The results are representative of at least three independent experiments.