Figure 2

Capsaicin treatment results in apoptosis of KB cells. Apoptosis was assessed by Hoechst staining as described in the Methods. (A) The morphology of KB cells exposed to capsaicin was observed under a fluorescence microscope. Red arrows indicate apoptotic bodies. (B) The number of apoptotic cells was counted among at least 200 cells, and the percentage of apoptotic cells was charted. Data are representative of three independent experiments and are presented as the means ± SE of three independent experiments. Scale bar, 100 μm.