AAD prevented AHR in chronic OVA-challenged mice. Airway hyperresponsiveness (AHR) of mice was determined in the means of response to methacholine on 24 h after the last OVA (or NS) challenge by using the Buxco system as described in the Methods section. Data are presented as means ± SE for 8–10 mice in each group of three independent experiments. H + OVA: OVA challenged and treated with high dose AAD, L + OVA: OVA challenged and treated with low dose AAD. *P < 0.05 compared with the data of OVA group.