Curcumin regulates gene expression of insulin like growth factor, B-cell CLL/lymphoma 2 and antioxidant enzymes in streptozotocin induced diabetic rats

Table 1 Details giving primer sequences and expected product size for the genes amplified

cDNA	Sequence	Annealing temp.	Number of cycles	RT-PCR product size
β-actin F	5^/-AGC CAT GTA CGT AGC CAT CC-3^/	55	40	230
β-actin R	5^/- CTC TCA GCT GTG GTG GTG AA-3^/	55	40	230
IGF1F	5^/- CTG GGT GTC CAA ATG TAA CT-3^/	52	40	170
IGF1R	5^/-GTA TCT TTA TTG GAG GTG CG-3^/	52	40	170
SODF	5^/- AGG ATT AAC TGA AGG CGA GCA T-3^/	55	40	410
SODR	5^/- TCT ACA GTT AGC AGG CCA GCA G-3^/	55	40	410
CATF	5^/-ACG AGA TGG CAC ACT TTG ACA G −3^/	55	40	341
CATR	5^/-TGG GTT TCT CTT CTG GCT ATG G-3^/	55	40	341
GPxF	5^/-AAG GTG CTG CTC ATT GAG AAT G-3^/	57	40	406
GPxR	5^/-CGT CTG GAC CTA CCA GGA ACT T-3^/	57	40	406
Bcl-2 F	5^/- TCC ATT ATA AGC TGT CAC AGA GG-3^/	55	40	350
Bcl-2R	5^/- GAA GAG TTC CTC CAC CAC C-3^/	55	40	350
GSTF	5^/- GCT GGA GTG GAG TTT GAA GAA-3^/	55	40	575
GSTR	5/- GTC CTG ACC ACG TCA ACA TAG-3^/	55	40	575

ISSN: 2662-7671